Thanks for all this information! Some of it I knew, some of it was speculation, and some I had already but wasn't sure if it was right.
I've also found down the street in 1850 another Isaac 5 years younger who married a Sarah Wallisen so I've had to double check all my research and records to make sure I've got the right ones when I find them.
I do, however, believe that Ambrose was not Julius's father for the fact that Julius's grave stone indicates he was born October 29, 1787 and Ambrose has a Daughter, Lucy, born in March of 1787. I've found the Julius Hoffman family placed in three different family trees over the internet. The only one I've found the least contradictions is with Henry Hoffman and Elizabeth Blankenbacker. Most sources just list Julius as a son and nothing else.
What I do find curious, however, is Julius lives in clearcreek ohio surrounded by a clan of Huffmans from PA whereas Julius is from VA. At most, they're distant cousins from a brother of John Hoffman from Germany.