Jesse Rountree's will & inventory is filed with the North Carolina Secretary of State Loose Wills, Will of Jesse Rountree of Craven County. The inventory is signed by John Frizzle & John Rountree. His will is dated Oct 1777, filed with the state in Nov 1777.
Jesse Rountree mentioned his wife in his will, but not by name. Her name comes from this deed:
Pitt Co NC Deed Book F, pp. 203 - 204, deed of Jesse Rountree & wife Rachel Rountree of Craven Co NC to Randol McDonald of Pitt County NC. Deed dated 7 Aug 1776.
Rachel Rountree was also listed with William & John Rountree on the 1779 Craven Co NC tax list.
Of course, this just proves that Jesse's wife in 1776 and at his death in 1777 was named 'Rachel'. I have no record that she was the mother of all of his children.