1. Identify the DNA profile ("haplotype") of the immigrant ancestor John Blackwelder (Johannes Schwarzwalder), who came to America on 20 September 1738 (arriving in Philadelphia). His sons by his wife Elisabetha Maushardt, John Adam Blackwelder (Johann Adam Schwarzwalder) and Caleb Blackwelder (Gottlieb Schwarzwalder), born in Durrn, Wurttemberg, Germany on 30 September 1719 and 8 November 1722, respectively, moved to what is now Cabarrus County, North Carolina by the early 1760s. These two sons are believed to be the direct ancestors of most of the Blackwelder individuals in the USA today. By analyzing the Y-chromosome DNA of living Blackwelder descendants, it may also be possible (through minor mutations of the markers through the next 7+ generations) to be able to determine to which branch of this Blackwelder family a living individual belongs. This is important as John Adam Blackwelder's children and grandchildren are insufficiently documented, in contrast to his brother Caleb's immediate family.
2. Determine if any Blackwelders living in the USA today are not descended from the two sons of John Blackwelder (Johannes Schwarzwalder). Some Blackwelders are of African ancestry, descended from slaves of the Blackwelder families in the south who took the Blackwelder surname when they were emancipated after 1863.
3. Establish the relationship of John Blackwelder (Johannes Schwarzwalder) to other families of the Schwarzwalder surname (and its variants) in Germany. He was born in Monchweiler, Wurttemberg, Germany on 29 January 1684. His ancestry has been traced back to his great-grandfather Jacob Schwarzwalder, who died in Burgberg, Wurttemberg, Germany on 27 Jun 1646.