I assume that the witness, Mary Turk, was Mary Gleaves, second wife of Thomas Turk.I assume that the witness John Reaugh was John Rhea/Reah, husband of Margaret Turk (daughter of Thomas Turk and Mary Gleaves).I do not know who the witness Margaret Kirk was, however, I have a copy of the actual Will and her name is unmistakenly written as Kirk (with a dot over the "i".
My guess is that this John Mitchell, who wrote his Will in 1780 was going into the Revolutionary War as a young unmarried and childless man.He describes himself as "being at the present of sound memory and perfect health".The "perfect health" is unusual language for a Will, particularly of an older person whose health is failing.His Will was proved in 1783 showing that he survived about 3 years after writing his Will.
Also, there is a John Mitchell who resided in Augusta Co., VA, who wrote his Will in 1771 which was proved in 1771 which mentions several children, including a John Mitchell, his third son, who was devised some land.I wonder if the John Mitchell who died in 1783 was the son of this John Matchell.The older John Mitchell's Will does not mention a Russell, Turk, Kirk or Gleaves.Of course, there may be no relationship between these two John Mitchells.
It is my educated guess that John Reaugh, Mary Turk and Margaret Kirk were more than passing travelers to John Mitchell when he signed his Will in 1780.I think John Mitchell was well acquainted with at least one of them.Where the signing took place and what brought them all together at that time I cannot say.
I doubt that John Mitchell who died in 1783 could have been old enought to be Esther __?__ (Turk/Rafferty/Woolman's) father.Perhaps brother.Maybe nephew or simply a neighbor or related church-goer.I wonder if 23 Sept. 1780 was a Sunday?
Regarding the John Mitchell who turned in a wolf's head in 1743, I do not see how we can tell which John Mitchell this reference is referring to.Also, 1743 is 2 years before there is a reference of Matthew Gleaves marrying in Philadelphia, PA.If our Matthew Gleaves, Sr., married in Philadelphia in 1745, it seems unlikely that he would have been marrying a daughter of John Mitchell who was living in Orange Co., VA in 1743.
I think we need something more concrete to go on before concluding that there is more than a remote possibility that John Mitchell (or any Mitchell) is the father of Esther who married Matthew Gleaves, Sr.