This may be a very long shot... We have Josephina Heyne born 18 oct 1851 died 9 may 1930 in Carthagena, mercer co., Ohio. She married in Dec 1877 in Fort Larmie, Shelby co., Ohio to Joseph Bernard Barhorst, son of Franz Barhorst and 2nd wife Maria Elisabeth Giere.
I have never seen any mention of her parents. Have you any more info. on that missing brother in your line?