I am rather confused by your last posting as some things are not too clear in it: Which Samuel (senior or junior) married Jane? Similarily have you got Robert or Samuel married to Isabel Firth? The details I have copied from "All Saints Parish Church of Bingley" Records give "Samuell Longbothu" marrying "Isabell Firth" on 4th Apr 1620. "Robert Longbothu" married "Isabell Hardcastle" on 11th Nov 1634 not 1635!
I can find no records early enough to confirm that Samuel married a Jane nor a date of birth for him arounf 1573. Can you please advise as to your source for this information?
Was is Stephen or John who married Martha Rogers as I can find no detail in my copies of such a marriage? I could have missed it!
With regards to Roberts two sons John and Robert. I do not have a Robert. I do have the following all shown as the father residing at Eldwick (variously spelt "Helweeke", "Helweek", "Helwik" or "Helw'"): Baptisms of "John Longbothu'" 15th Nov 1635; "Richard Longbothu" 19th Nov 1637; "Samuell Longbothu" 3rd May 1640; Isabell Longbothome" 4th Sep 1642; "Martha Longbothum" 9th Feb 1644; "John Longbothom" 30th Oct 1652 (Father's occupation given as "Taylor for this entry only); "Mary Longbothum" 18th Feb 1654. Two further baptism entries for a father Robert residing in Eldwick are given in 1681 and 1687 but these dates suggest a different family.
Hope these help. Look forward to your responses to the queries raised above.