Another follow-up. John is listed in Greene 1840, p 116. His wife Dorcas is listed in the 1850 deaths, "Died of grief" in March 1850 -- Her son George died in Feb 1850, Reuben in April. Your Mary Ann is married to John Holmes 1850 Indiana, Lawrence Co, Perry Twp, p461.
Sisters Ludica and Dorcas are living with Orrin and Barbary Tally (from NC) in Greene Co, Eel River Twp, p.401 in 1850, Elizabeth (probably) living with Jeremiah and Sally Wakefield in Greene Co, Stockton Twp, p.363. So her father John Sr, and brothers James and John are missing.