Re: John Penn Signed the Decelaration of Independance
Jeff Penn of Chinqua Penn lived later than your Charles Penn, so the Penns in your line might be distant cousins, but do not appear to be more closely related. Jeff's line is somewhat close to my own line (we're double cousins several times removed due to a Penn son who married his Kennerly stepmother's sister). This line was in Virginia well after Charles was born in Ohio. (My Penn grandmother was born in Columbus, Ohio, in 1919, but her father had been born in Virginia in 1880.)
As it happens, this is not the line of John Penn, but of his brother Abram, who was a colonel in the Virginia militia at the same time John was representing North Carolina in the Continental Congress. Someone will correct me if I'm mistaken, but I believe that none of John Penn's male children lived to adulthood.
I am curious as to why you think your Penns are connected to the Virginia branch. I notice you have Virgnia roots on some matrilineal lines, and it's not unusual to find Virginians in southern Ohio in the early 1800s. But it's equally common to find Pennsylvanians there and Penn is obviously a well-established name in Pennsylvania. I would think that connection would be more likely.
No relationship between the Pennsylvania and Virginia Penns has ever been proven to my knowledge. If there is a connection between the families, it would most likely be in generations that lived on the opposite side of the Atlantic.
Good luck in your research.